1MC8
Crystal Structure of Flap Endonuclease-1 R42E mutant from Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2000-11-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 |
| Unit cell lengths | 62.670, 62.670, 180.690 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 3.100 |
| R-factor | 0.197 |
| Rwork | 0.190 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a76 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 3.100 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.700 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.104 | 0.494 |
| Total number of observations | 135478 * | |
| Number of reflections | 15903 | |
| Completeness [%] | 100.0 | 98.6 |
| Redundancy | 5.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | PEG6000, sodium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2 (%) | |
| 2 | 1 | drop | 200 (mM) | pH8.0 | |
| 3 | 1 | drop | Tris-HCl | 50 (mM) | pH6.0 |
| 4 | 1 | reservoir | PEG6000 | 20 (%) | pH6.0 |
| 5 | 1 | reservoir | MES | 100 (mM) |
