1MAU
Crystal structure of Tryptophanyl-tRNA Synthetase Complexed with ATP and Tryptophanamide in a Pre-Transition state Conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.133, 62.133, 217.807 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| R-factor | 0.212 |
| Rwork | 0.208 |
| R-free | 0.24400 * |
| Structure solution method | SIR |
| Starting model (for MR) | 1m83 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.975 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.079 * | 0.320 * |
| Total number of observations | 335452 * | |
| Number of reflections | 23318 | |
| <I/σ(I)> | 16 | 2.8 |
| Completeness [%] | 90.5 | 77.5 |
| Redundancy | 5.5 | 2.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.5 | 35 * | sodium citrate, magnesium chloride, sodium ATP , l-tryptophanamide, pH 7.5, MICRODIALYSIS, temperature 310K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 4 (mg/ml) | |
| 2 | 1 | 1 | potassium phosphate | 0.6 (M) | |
| 3 | 1 | 1 | glycerol | 50 (%(v/v)) | |
| 4 | 1 | 2 | sodium citrate | 1 (M) | |
| 5 | 1 | 2 | ATP | 10 (mM) | |
| 6 | 1 | 2 | 10 (mM) | ||
| 7 | 1 | 2 | tryptophanamide | 50000 (mM) |
