1MAT

STRUCTURE OF THE COBALT-DEPENDENT METHIONINE AMINOPEPTIDASE FROM ESCHERICHIA COLI: A NEW TYPE OF PROTEOLYTIC ENZYME

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	39.000, 61.700, 54.500
Unit cell angles	90.00, 107.30, 90.00

Refinement procedure

Resolution	52.000 * - 2.500 *
R-factor	0.182
RMSD bond length	0.019
RMSD bond angle	2.900
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.035 *
Number of reflections	8054 *
Completeness [%]	93.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	42 (mg/ml)
2	1	drop	sodium cacodylate	25 (M)
3	1	drop		25 (mM)
4	1	drop		100 (mM)
5	1	drop		1 (mM)
6	1	drop	PEG4000	14 (%(w/v))
7	1	drop	octyl beta-glucoside	0.5 (%(w/v))
8	1	reservoir	PEG4000	23-35 (%)