1M93
1.65 A Structure of Cleaved Viral Serpin CRMA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-02-12 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.520, 92.590, 100.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.540 - 1.650 |
Rwork | 0.188 |
R-free | 0.24540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 1C8O |
RMSD bond length | 0.009 |
RMSD bond angle | 0.027 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.540 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.039 | 0.470 |
Number of reflections | 53225 | |
<I/σ(I)> | 32.6 | 2.1 |
Completeness [%] | 94.2 | 83.5 |
Redundancy | 5.6 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | sodium/potassium phosphate 1.6M, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |