1M7W

HNF4a ligand binding domain with bound fatty acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Detector	BRANDEIS - B1.2
Spacegroup name	P 41 21 2
Unit cell lengths	102.288, 102.288, 227.688
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
Rwork	0.237
R-free	0.28200
Structure solution method	MAD
RMSD bond length	0.008 *
RMSD bond angle	1.350 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800
Number of reflections	29727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8 *		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	reservoir	sodium citrate	0.1 (M)	pH8.0
3	1	reservoir	ammonium acetate	0.7 (M)
4	1	reservoir	MPD	16 (%)
5	1	reservoir	dithiothreitol	10 (mM)