1M7E

Crystal structure of the phosphotyrosine binding domain(PTB) of mouse Disabled 2(Dab2):implications for Reeling signaling

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-09-30
Detector	MAC Science DIP-2000
Wavelength(s)	1.5418
Spacegroup name	H 3 2
Unit cell lengths	127.258, 127.258, 269.541
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.450
Rwork	0.244
R-free	0.27200 *
Structure solution method	SIR
RMSD bond length	0.007
RMSD bond angle	1.210 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.500
High resolution limit [Å]	2.450	2.450
Rmerge	0.100 *
Total number of observations	712193 *
Number of reflections	31089
<I/σ(I)>	21.6
Completeness [%]	98.7	88.2
Redundancy	22.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	18 *	sodium formate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	20 (mM)	pH7.5
2	1	drop	dithiothreitol	1 (mM)
3	1	drop	EDTA	1 (mM)
4	1	drop	protein	20 (mg/ml)
5	1	reservoir	sodium formate	3.8 (M)
6	1	reservoir	HEPES	50 (mM)	pH8.5