1M6I

Crystal Structure of Apoptosis Inducing Factor (AIF)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	110
Detector technology	CCD
Collection date	2001-09-24
Wavelength(s)	0.9997
Spacegroup name	P 1 21 1
Unit cell lengths	50.900, 90.500, 60.500
Unit cell angles	90.00, 94.60, 90.00

Refinement procedure

Resolution	40.000 * - 1.800
Rwork	0.225
R-free	0.24200 *
Structure solution method	SOLVE
RMSD bond length	0.006 *
RMSD bond angle	1.300 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *
High resolution limit [Å]	1.800
Rmerge	0.062 *	0.264 *
Number of reflections	44620
Completeness [%]	99.4 *	99.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8	298	PEG8000, DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	6 (mg/ml)
2	1	2	PEG8000	16 (%(w/v))
3	1	2	dithiothreitol	5 (mM)
4	1	2	Tris	100 (mM)	pH8.0