1M6B

Structure of the HER3 (ERBB3) Extracellular Domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Detector technology	CCD
Spacegroup name	C 1 2 1
Unit cell lengths	236.260, 49.620, 190.860
Unit cell angles	90.00, 125.56, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.2381
Rwork	0.235
R-free	0.29400 *
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.590 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.600
Rmerge	0.067 *	0.521 *
Number of reflections	48669
Completeness [%]	96.3 *	79.2 *
Redundancy	3.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	pH 9.0, VAPOR DIFFUSION, HANGING DROP at 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	reservoir	PEG4000	15-20 (%)
3	1	reservoir	Tris	0.1 (M)	pH9.0
4	1	reservoir	lithium sulfate	0.15 (M)