1M5R
Ternary complex of T4 phage BGT with UDP and a 13 mer DNA duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.000, 55.900, 99.500 |
| Unit cell angles | 90.00, 91.10, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.191 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jg6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.180 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.068 * | |
| Number of reflections | 83161 | |
| <I/σ(I)> | 19.1 | 2.4 |
| Completeness [%] | 98.1 | 95.7 |
| Redundancy | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | Lariviere, L., (2002) Acta Crystallogr., Sect.D, 58, 1484. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | tert-butanol | ||
| 3 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 50 (mM) | |
| 2 | 1 | drop | BGT | 50 (mM) | |
| 3 | 1 | drop | UDP-glucose | 0.5 (mM) | |
| 4 | 1 | drop | MPD | 22 (%) | |
| 5 | 1 | drop | t-butanol | 2 (%) | |
| 6 | 1 | reservoir | MPD | 44 (%) | |
| 7 | 1 | reservoir | t-butanol | 4 (%) |
