1M5P
Transition State Stabilization by a Catalytic RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 150 |
Detector technology | IMAGE PLATE |
Collection date | 2001-12-12 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 259.800, 44.300, 102.400 |
Unit cell angles | 90.00, 107.00, 90.00 |
Refinement procedure
Resolution | 27.700 - 2.600 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1HP6 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 34446 | |
<I/σ(I)> | 22 | 1.7 |
Completeness [%] | 98.8 | 96.4 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 300 | MPD, calcium Chloride, ammonium chloride, meta-ammonium vanadate, pH 5.0, VAPOR DIFFUSION, SITTING DROP at 300K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | CaCl2 | ||
2 | 1 | 1 | NH4Cl | ||
3 | 1 | 1 | meta-ammonium vanadate | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 2 | CaCl2 | ||
6 | 1 | 2 | NH4Cl | ||
7 | 1 | 2 | MPD |