1M3J
CRYSTAL form II of perfringolysin O
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 167.000, 214.110, 47.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
Rwork | 0.247 |
R-free | 0.33700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pfo |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 3.000 |
Rmerge | 0.151 |
Number of reflections | 24516 |
Completeness [%] | 70.5 |
Redundancy | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |