1M3I

Perfringolysin O, new crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-10-01
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 31
Unit cell lengths	130.410, 130.410, 129.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.900
Rwork	0.233
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pfo
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.061	0.571
Number of reflections	59700
<I/σ(I)>	16.8
Completeness [%]	97.8	97.4
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	298	8% peg 8000, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K