1M34
Nitrogenase Complex From Azotobacter Vinelandii Stabilized By ADP-Tetrafluoroaluminate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.005 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 326.100, 75.800, 312.200 |
| Unit cell angles | 90.00, 102.60, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| Rwork | 0.200 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.630 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.128 * | 0.266 * |
| Total number of observations | 665961 * | |
| Number of reflections | 273781 | |
| Completeness [%] | 83.3 | 66.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.5 | 4 * | PEG 8000, cacodylate, magnesium chloride, pH 6.5, LIQUID DIFFUSION, temperature 296.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 15 (mg/ml) | |
| 10 | 1 | 2 | 30 (mM) | ||
| 11 | 1 | 2 | PEG8000 | 19 (%(w/w)) | |
| 2 | 1 | 1 | MOPS/Tris | 100 (mM) | pH7.3 |
| 3 | 1 | 1 | 100 (mM) | ||
| 4 | 1 | 1 | 5 (mM) | ||
| 5 | 1 | 1 | 0.25 (mM) | ||
| 6 | 1 | 1 | 1 (mM) | ||
| 7 | 1 | 1 | 1 (mM) | ||
| 8 | 1 | 1 | 5 (mM) | ||
| 9 | 1 | 2 | cacodylate-HCl | 100 (mM) | pH6.5 |
