1M34
Nitrogenase Complex From Azotobacter Vinelandii Stabilized By ADP-Tetrafluoroaluminate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.005 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 326.100, 75.800, 312.200 |
Unit cell angles | 90.00, 102.60, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
Rwork | 0.200 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.630 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.128 * | 0.266 * |
Total number of observations | 665961 * | |
Number of reflections | 273781 | |
Completeness [%] | 83.3 | 66.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 6.5 | 4 * | PEG 8000, cacodylate, magnesium chloride, pH 6.5, LIQUID DIFFUSION, temperature 296.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 15 (mg/ml) | |
10 | 1 | 2 | 30 (mM) | ||
11 | 1 | 2 | PEG8000 | 19 (%(w/w)) | |
2 | 1 | 1 | MOPS/Tris | 100 (mM) | pH7.3 |
3 | 1 | 1 | 100 (mM) | ||
4 | 1 | 1 | 5 (mM) | ||
5 | 1 | 1 | 0.25 (mM) | ||
6 | 1 | 1 | 1 (mM) | ||
7 | 1 | 1 | 1 (mM) | ||
8 | 1 | 1 | 5 (mM) | ||
9 | 1 | 2 | cacodylate-HCl | 100 (mM) | pH6.5 |