1M2T
Mistletoe Lectin I from Viscum album in Complex with Adenine Monophosphate. Crystal Structure at 1.9 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-09-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8459 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 107.120, 107.120, 309.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.890 |
| R-factor | 0.2101 |
| Rwork | 0.210 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | unpublished structure |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.029 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.930 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.068 | 0.764 |
| Number of reflections | 83270 | |
| <I/σ(I)> | 22 | 2.3 |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 38 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapor diffusion under microgravity | 2.5 | 293 | ammonium sulphate, pH 2.5, vapor diffusion under microgravity at 293K |
