1M2G
Sir2 homologue-ADP ribose complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6B |
Synchrotron site | PAL/PLS |
Beamline | 6B |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Detector | MAC Science DIP-2030 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.917, 85.512, 44.854 |
Unit cell angles | 90.00, 112.34, 90.00 |
Refinement procedure
Resolution | 40.000 * - 1.470* |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.23100 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.160 * |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 * |
High resolution limit [Å] | 1.470 * |
Rmerge | 0.031 * |
Total number of observations | 98112 * |
Number of reflections | 36883 * |
Completeness [%] | 88.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 | 18 * | Sodium Citrate, PEG 4000, pH 6.0, EVAPORATION, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mM) | |
2 | 1 | reservoir | sodium citrate | 100 (mM) | pH6.0 |
3 | 1 | reservoir | PEG4000 | 14 (%) | |
4 | 1 | reservoir | isopropanol | 20 (%) |