1M1L
Human Suppressor of Fused (N-terminal domain)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-05-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 173.025, 173.025, 290.833 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.810 - 2.650 |
Rwork | 0.224 |
R-free | 0.25600 * |
Structure solution method | SAD |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 * | |
High resolution limit [Å] | 2.650 | 2.650 * |
Rmerge | 0.094 * | 0.362 * |
Total number of observations | 441505 * | |
Number of reflections | 48643 | |
Completeness [%] | 99.2 | 100 * |
Redundancy | 9.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 279 | 1 M LiCl, 0.1 M NaCitrate pH 5.0- 6.0, 10% w/v PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2.5 (mg/ml) | |
2 | 1 | drop | Tris | 25 (mM) | pH7.5 |
3 | 1 | drop | dithiothreitol | 10 (mM) | |
4 | 1 | reservoir | 1 (M) | ||
5 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.0-6.0 |
6 | 1 | reservoir | PEG6000 | 10 (%(w/v)) |