1M1E

Beta-catenin armadillo repeat domain bound to ICAT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 21 21 2
Unit cell lengths	97.750, 97.320, 86.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.560 - 2.100
R-factor	0.202
Rwork	0.202
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i7w
RMSD bond length	0.005
RMSD bond angle	18.300 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	COMO
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.059 *	0.250 *
Number of reflections	47814
<I/σ(I)>	7.8	4.7
Completeness [%]	96.6	97.7
Redundancy	3.4	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	22 *	MES, MPD, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	MES	100 (mM)	pH6.5
3	1	reservoir	dithiothreitol	5 (mM)
4	1	reservoir	MPD	1-2 (%(v/v))