1M0I
Crystal Structure of Bacteriophage T7 Endonuclease I with a Wild-Type Active Site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-03-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 123.440, 134.550, 61.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.130 - 2.550 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m0d |
RMSD bond length | 0.011 |
RMSD bond angle | 1.600 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.130 | 2.680 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 34014 | |
<I/σ(I)> | 9.5 | 2.4 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 4.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop. Seeds of E65K mutant used. | 7.2 | 291 | PEG 4000, Ammonium sulphate, Sodium chloride, Tris HCL, pH 7.2, Vapor diffusion, hanging drop. Seeds of E65K mutant used., temperature 291K |