1M0D
Crystal Structure of Bacteriophage T7 Endonuclease I with a Wild-Type Active Site and Bound Manganese Ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 123.810, 133.960, 61.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.800 - 1.900 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fzr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.380 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.550 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.085 * | |
| Total number of observations | 419157 * | |
| Number of reflections | 81257 | |
| <I/σ(I)> | 7.6 | 1.6 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 5.2 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 291 | PEG 4000, Ammonium sulphate, Sodium chloride, Tris HCL, pH 7.2, Vapor diffusion, hanging drop. Seeds of E65K mutant used, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | protein | 4 (mg/ml) | |
| 4 | 1 | reservoir | PEG4000 | 17-19 (%) | |
| 5 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.2 |
