1LZV
Site-Specific Mutant (Tyr7 replaced with His) of Human Carbonic Anhydrase II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2002-04-10 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.548 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.871, 41.585, 72.951 |
Unit cell angles | 90.00, 104.75, 90.00 |
Refinement procedure
Resolution | 19.940 - 2.300 |
Rwork | 0.186 |
R-free | 0.24800 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g0f |
RMSD bond length | 0.006 |
RMSD bond angle | 1.366 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.151 | 0.401 * |
Number of reflections | 9586 * | 979 * |
Completeness [%] | 85.0 | 87.3 * |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 40 * | Ammonium Sulfate, Tris HCL, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.8 |
3 | 1 | reservoir | ammonium sulfate | 2.4-2.65 (M) | |
4 | 1 | reservoir | Tris-HCl | 50 (mM) | pH7.8 |