1LY0
Structure of thaumatin crystallized in the presence of glycerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-12-17 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9474 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 57.867, 57.867, 149.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.360 |
R-factor | 0.196 |
Rwork | 0.195 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1thw |
RMSD bond length | 0.005 |
RMSD bond angle | 1.308 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.410 |
High resolution limit [Å] | 1.360 | 1.360 |
Number of reflections | 55482 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | tartrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |