1LX5

Crystal Structure of the BMP7/ActRII Extracellular Domain Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.03
Spacegroup name	P 62 2 2
Unit cell lengths	140.920, 140.920, 90.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	100.000 * - 3.300
R-factor	0.24
Rwork	0.238
R-free	0.27900
Structure solution method	MIR
RMSD bond length	0.032
RMSD bond angle	2.978
Data reduction software	TRUNCATE
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	MLPHARE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *	3.420
High resolution limit [Å]	3.300	3.300
Rmerge	0.055 *	0.332 *
Total number of observations	31531 *
Number of reflections	8320
<I/σ(I)>	16.5
Completeness [%]	96.7	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	23 *	1M sodium acetate, 0.1M imidazole, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 296K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	sodium acetate	1 (M)
3	1	reservoir	imidazole	100 (mM)	pH7.