1LWU
Crystal structure of fragment D from lamprey fibrinogen complexed with the peptide Gly-His-Arg-Pro-amide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1998-08-22 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.735, 47.654, 244.650 |
| Unit cell angles | 88.81, 97.23, 86.17 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.251 |
| Rwork | 0.245 |
| R-free | 0.28900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.380 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.078 | |
| Total number of observations | 200629 * | |
| Number of reflections | 74102 | |
| Completeness [%] | 88.3 | 58.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 295 | PEG 3350, MES buffer, pH 6.75, 10 mM CaCl2, VAPOR DIFFUSION, SITTING DROP at 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | lamprey fragment D | 6-8 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH7.0 |
| 3 | 1 | drop | GHRPam | 4 (mM) | |
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | reservoir | PEG | 12 (%) | |
| 6 | 1 | reservoir | MES | 50 (mM) | pH6.5 |
| 7 | 1 | reservoir | 20 (mM) |
