1LWO
Crystal structure of rabbit muscle glycogen phosphorylase a in complex with a potential hypoglycaemic drug at 2.0 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2000-03-03 |
Wavelength(s) | 1.05 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.648, 128.648, 116.521 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.400 * - 2.000 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.23500 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1lwn |
RMSD bond length | 0.007 |
RMSD bond angle | 1.260 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.078 | 0.658 |
Total number of observations | 343075 * | |
Number of reflections | 64780 * | 3121 * |
<I/σ(I)> | 13.2 | 3.3 |
Completeness [%] | 97.5 | 95.7 |
Redundancy | 4.8 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 298 | Oikonomakos, N.G., (1999) Protein Sci., 8, 1930. * |