1LW5
X-ray structure of L-Threonine Aldolase (low-specificity) in complex with glycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2001-07-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9690 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.020, 100.710, 150.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 * |
| R-factor | 0.2088 |
| Rwork | 0.207 |
| R-free | 0.23700 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.120 |
| High resolution limit [Å] | 2.000 * | 2.050 |
| Rmerge | 0.100 * | 0.279 * |
| Total number of observations | 903510 * | |
| Number of reflections | 86689 | |
| Completeness [%] | 95.1 | 91.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | glycine,PEG400, calcium chloride, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 30 (%(v/v)) | |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
| 4 | 1 | reservoir | TCEP | 0.5 (mM) |
