1LVW
Crystal structure of glucose-1-phosphate thymidylyltransferase, RmlA, complex with dTDP
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-12-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0719, 1.0726, 1.0455 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 112.680, 115.880, 116.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.520 - 1.700 |
Rwork | 0.187 |
R-free | 0.21500 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 161361 | |
<I/σ(I)> | 19.4 | 2.5 |
Completeness [%] | 96.2 | 71.6 |
Redundancy | 4.15 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 2.0 M ammonium sulfate, 0.1 M sodium acetate, 24% glycerol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |