1LV0
Crystal structure of the Rab effector guanine nucleotide dissociation inhibitor (GDI) in complex with a geranylgeranyl (GG) peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 98 |
Detector technology | CCD |
Collection date | 2000-02-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.033 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.230, 42.861, 89.104 |
Unit cell angles | 90.00, 103.53, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.000 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1d5t |
RMSD bond length | 0.005 * |
RMSD bond angle | 23.800 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.061 | 0.419 |
Total number of observations | 68840 * | |
Number of reflections | 30213 | |
<I/σ(I)> | 12.7 | 1.4 |
Completeness [%] | 95.7 | 85.9 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.25 * | 22.5 * | Luan, P., (2000) Traffic, 1, 270., Schalk, I., (1996) Nature,381, 42. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 1.73 (M) |