1LU5
2.4 Angstrom Crystal Structure of the Asymmetric Platinum Complex {Pt(ammine)(cyclohexylamine)}2+ Bound to a Dodecamer DNA Duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.644, 35.638, 46.556 |
| Unit cell angles | 79.53, 83.02, 81.36 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-free | 0.24950 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aio |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.022 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 * | 0.277 * |
| Total number of observations | 42245 * | |
| Number of reflections | 7337 | |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 96.3 | 96.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4. * | spermine, sodium cacodylate, magnesium acetate, PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | spermine | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | magnesium acetate | ||
| 4 | 1 | 1 | PEG 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.2 (mM) | |
| 2 | 1 | reservoir | 50 (mM) | pH6.5 | |
| 3 | 1 | reservoir | spermine | 12 (mM) | |
| 4 | 1 | reservoir | 120 (mM) | ||
| 5 | 1 | reservoir | PEG4000 | 15 (%(w/v)) |
