1LTK

CRYSTAL STRUCTURE OF PHOSPHOGLYCERATE KINASE FROM PLASMODIUM FALCIPARUM, IN THE OPEN CONFORMATION

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-17
Detector	MARRESEARCH
Wavelength(s)	0.9537, 0.9792, 0.9793
Spacegroup name	I 2 2 2
Unit cell lengths	119.030, 147.610, 206.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.930 - 3.000
R-factor	0.2845
Rwork	0.208
R-free	0.25800
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.800
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE (VERSION 1.18)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Number of reflections	36637
<I/σ(I)>	11.3
Completeness [%]	99.8	100
Redundancy	10.4	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	HEPES, AMMONIUM SULFATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	HEPES, AMMONIUM SULFATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	HEPES, AMMONIUM SULFATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K