1LS3

Crystal Structure of the Complex between Rabbit Cytosolic Serine Hydroxymethyltransferase and TriGlu-5-formyl-tetrahydrofolate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2000-12-07
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 41
Unit cell lengths	114.084, 114.084, 156.719
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.700
R-factor	0.22
Rwork	0.220
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2.1 Angstrom Structure of Rabbit Cytosolic Serine Hydroxymethyltransferase
RMSD bond length	0.007
RMSD bond angle	22.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.080 *	0.520 *
Number of reflections	48537 *
<I/σ(I)>	9.9	1.67
Completeness [%]	87.0 *	94 *
Redundancy	2.2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7	298	used seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	50 (mg/ml)
2	1	1	PEG4000	3 (%)
3	1	1	potassium MES	50 (mM)	pH7.0