1LRY
Crystal Structure of P. aeruginosa Peptide Deformylase Complexed with Antibiotic Actinonin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 1997-10-22 |
Detector | MAC Science DIP-2000 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.430, 60.130, 73.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.600 |
R-factor | 0.221 |
Rwork | 0.221 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.088 * | 0.246 * |
Number of reflections | 7756 | |
Completeness [%] | 85.5 | 85.3 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 19 * | 32% PEG6000, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | CAPS-HCl | 30 (mM) | pH9.5 |
2 | 1 | drop | PEG6000 | 16 (%) | |
3 | 1 | drop | protein | 0.6 (mM) | |
4 | 1 | drop | actinonin | 0.6 (mM) | |
5 | 1 | reservoir | CAPS-HCl | 60 (mM) | pH9.5 |
6 | 1 | reservoir | PEG6000 | 32 (%) |