1LRU

Crystal Structure of E.coli Peptide Deformylase Complexed with Antibiotic Actinonin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	1997-04-14
Detector	MAC Science DIP-2000
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	141.040, 63.608, 83.494
Unit cell angles	90.00, 123.35, 90.00

Refinement procedure

Resolution	15.000 - 2.100
R-factor	0.24
Rwork	0.240
Structure solution method	SIRAS
RMSD bond length	0.012
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.076 *	0.251 *
Number of reflections	34394
Completeness [%]	93.7	83.7
Redundancy	2.4	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	19 *	0.5M (NH4)2SO4, 28%PEG400, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MES-NaOH	25 (mM)	pH6.0
2	1	drop	ammonium sulfate	1.0 (M)
3	1	drop	protein	0.5 (mM)
4	1	drop	actinonin	0.5 (mM)
5	1	reservoir	MES-NaOH	50 (mM)	pH6.0
6	1	reservoir	ammonium sulfate	2.0 (M)