1LRJ
Crystal Structure of E. coli UDP-Galactose 4-Epimerase Complexed with UDP-N-Acetylglucosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 115 |
| Detector technology | AREA DETECTOR |
| Collection date | 2001-11-28 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.200, 83.200, 108.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ek6 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 16.500 * |
| Data reduction software | FEAMBO |
| Data scaling software | SAINT |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.048 * | 0.356 * |
| Number of reflections | 42024 | 4851 * |
| <I/σ(I)> | 17 | 2 |
| Completeness [%] | 92.3 | 81.4 |
| Redundancy | 3.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 4 * | PEG 8000, NaCl, HEPES, UDP-N-acetylglucosamine, pH 8.0, VAPOR DIFFUSION, HANGING DROP at 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | potassium phosphate | 10 (mM) | pH8.0 |
| 2 | 1 | drop | protein | 25 (mg/ml) | |
| 3 | 1 | reservoir | PEG8000 | 15-19 (%) | |
| 4 | 1 | reservoir | 200 (mM) | ||
| 5 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
