1LR8
Crystal structure of Fs1, the heparin-binding domain of follistatin, complexed with the heparin analogue D-myo-inositol hexasulphate (Ins6S)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9202 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 21.499, 38.208, 77.945 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.900 * - 2.100 |
| R-factor | 0.22216 |
| Rwork | 0.221 |
| R-free | 0.25100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lr7 |
| RMSD bond length | 0.026 * |
| RMSD bond angle | 2.270 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 * | 0.260 * |
| Number of reflections | 4026 | |
| Completeness [%] | 98.6 | 99.7 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 18 * | 15-25% PEG8000, 0.2-0.6 M Magnesium acetate, 0.1 M Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 15-25 (%) | |
| 3 | 1 | reservoir | magnesium acetate | 0.2-0.6 (M) | |
| 4 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
