1LQV
Crystal structure of the Endothelial protein C receptor with phospholipid in the groove in complex with Gla domain of protein C.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.220, 62.360, 71.030 |
| Unit cell angles | 90.00, 101.81, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.600 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.20500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Endothelial protein C receptor |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.730 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.050 * | 0.455 |
| Number of reflections | 66375 | |
| <I/σ(I)> | 27 | 27 |
| Completeness [%] | 98.0 * | 83.8 * |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 * | 298 | peg 400, potassium chloride, magnesium chloride, calcium chloride, hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 10 (mM) | ||
| 10 | 1 | 2 | polyethylene glycol | 5 (%(v/v)) | |
| 2 | 1 | 1 | 0.02 (%) | ||
| 3 | 1 | 1 | protein | 20 (mg/ml) | |
| 4 | 1 | 1 | Tris-HCl | 20 (mM) | pH7.5 |
| 5 | 1 | 1 | 100 (mM) | ||
| 6 | 1 | 2 | 100 (mM) | ||
| 7 | 1 | 2 | 10 (mM) | ||
| 8 | 1 | 2 | 10 (mM) | ||
| 9 | 1 | 2 | HEPES | 50 (mM) | pH7.0 |
