1LQS
CRYSTAL STRUCTURE OF HUMAN CYTOMEGALOVIRUS IL-10 BOUND TO SOLUBLE HUMAN IL-10R1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-10-30 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 114.290, 104.680, 91.890 |
Unit cell angles | 90.00, 106.49, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.700 |
Rwork | 0.244 |
R-free | 0.29500 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j7v |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.350 * |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.056 * | 0.195 |
Total number of observations | 65855 * | |
Number of reflections | 26564 * | |
Completeness [%] | 92.5 * | 73.7 * |
Redundancy | 2.8 * | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 25 * | PEG-6000, 0.1M MgCl2, 100mM ADA, 0.75% PEG-400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris | 20 (mM) | pH8.0 |
3 | 1 | reservoir | ADA buffer | 100 (mM) | pH6.0 |
4 | 1 | reservoir | PEG6000 | 6 (%) | |
5 | 1 | reservoir | 0.1 (M) | ||
6 | 1 | reservoir | PEG400 | 0.75 (%) |