1LOS
crystal structure of orotidine monophosphate decarboxylase mutant deltaR203A complexed with 6-azaUMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-03-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 52.490, 56.152, 73.870 |
Unit cell angles | 82.76, 89.89, 76.91 |
Refinement procedure
Resolution | 30.000 * - 1.900 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 22.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.048 * | 0.283 * |
Total number of observations | 489020 * | |
Number of reflections | 58208 | |
Completeness [%] | 90.6 | 64 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | trisodium citrate, MgCl2, dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 25 (mM) | ||
2 | 1 | reservoir | dioxane | 5 (%) |