1LOK
The 1.20 Angstrom Resolution Crystal Structure of the Aminopeptidase from Aeromonas proteolytica Complexed with Tris: A Tale of Buffer Inhibition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-02-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 108.338, 108.338, 93.519 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 * - 1.200 |
| R-factor | 0.145 |
| Rwork | 0.158 |
| R-free | 0.17600 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1amp |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 1.250 |
| High resolution limit [Å] | 1.200 * | 1.200 * |
| Rmerge | 0.057 * | 0.290 * |
| Total number of observations | 1348266 * | |
| Number of reflections | 117248 * | |
| Completeness [%] | 99.0 * | 98 |
| Redundancy | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 | 295 | Tris, potassium thiocyanate, sodium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | pH8.0 |
| 3 | 1 | drop | KSCN | 10 (mM) | |
| 4 | 1 | drop | 0.4 (M) | ||
| 5 | 1 | reservoir | Tris | 100 (mM) | pH8.0 |
| 6 | 1 | reservoir | KSCN | 100 (mM) | |
| 7 | 1 | reservoir | 4.5 (M) |
