1LOC

INTERACTION OF A LEGUME LECTIN WITH TWO COMPONENTS OF THE BACTERIAL CELL WALL

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	56.580, 139.800, 63.400
Unit cell angles	90.00, 91.50, 90.00

Refinement procedure

Resolution	6.000 - 2.050
R-factor	0.189
Rwork	0.189
RMSD bond length	0.017
RMSD bond angle	3.200
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000 *
High resolution limit [Å]	2.050 *
Rmerge	0.069 *
Total number of observations	248619 *
Number of reflections	57554 *
Completeness [%]	97.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	20 *	reservoirs contain the same ingredients as drops, but twice as consentrated. taken from Bourne, Y. et al. (1988). J. Mol. Biol., 202, 685-687. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	MPD	40 (%(v/v))
3	1	drop		0.05 (M)
4	1	drop	HEPES	0.02 (M)