1LOA

THREE-DIMENSIONAL STRUCTURES OF COMPLEXES OF LATHYRUS OCHRUS ISOLECTIN I WITH GLUCOSE AND MANNOSE: FINE SPECIFICITY OF THE MONOSACCHARIDE-BINDING SITE

Experimental procedure

Spacegroup name	P 1 21 1
格子定数 [Å]	56.300, 139.800, 62.700
格子定数 [度]	90.00, 91.00, 90.00

精密化法

残基	8.000 - 2.200
R因子	0.179
Rwork	0.179
結合長の平均二乗偏差(RMSD) [Å]	0.003 *
結合角の平均二乗偏差(RMSD) [度]	3.000
Phasing software	X-PLOR
Refinement software	X-PLOR

Quality characteristics

	Overall
分解能 [Å] (高)	2.200 *
Rmerge_l_obs	0.082 *
Total number of observations	77034 *
独立反射数	33640 *
完全性 [%]	67.0 *

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Vapor diffusion, sitting drop *	7.5 *	20 *	reservoirs contain the same ingredients as drops, but twice as consentrated. taken from Bourne, Y. et al. (1988). J. Mol. Biol., 202, 685-687. *

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	10 (mg/ml)
2	1	drop	MPD	40 (%(v/v))
3	1	drop		0.05 (M)
4	1	drop	HEPES	0.02 (M)