1LLM

Crystal Structure of a Zif23-GCN4 Chimera Bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	133
Detector technology	IMAGE PLATE
Collection date	2000-06-08
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 31 2 1
Unit cell lengths	87.398, 87.398, 118.693
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.500
Rwork	0.216
R-free	0.23400
Structure solution method	MIR
RMSD bond length	0.015
RMSD bond angle	1.640 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.590
High resolution limit [Å]	1.500	1.500
Rmerge	0.095 *
Total number of observations	1187929 *
Number of reflections	83074 *
Completeness [%]	98.9 *	97.9
Redundancy	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	bis-tris propane, magnesium chloride, ammonium acetate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8	298	bis-tris propane, magnesium chloride, ammonium acetate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	bis-tris propane
2	1	1	MgCl2
3	1	1	ammonium acetate

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.9 (mM)
2	1	drop	Bis-Tris propane	80 (mM)	pH8.0
3	1	drop	DNA	0.056 (mM)
4	1	drop	Bis-Tris propane	10 (mM)	pH8.0
5	1	drop		1 (mM)
6	1	reservoir		600 (mM)