1LL0
Crystal Structure of Rabbit Muscle Glycogenin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-01 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795, 0.9794, 0.9322 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 139.460, 139.460, 416.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.430 |
| R-factor | 0.252 * |
| Rwork | 0.252 |
| R-free | 0.28600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 1.450 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.520 |
| High resolution limit [Å] | 3.430 | 3.430 |
| Rmerge | 0.088 | 0.484 |
| Total number of observations | 403861 * | |
| Number of reflections | 56152 | |
| <I/σ(I)> | 26 | 4.2 |
| Completeness [%] | 99.6 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 4 * | 8-10 mg/mL glycogenin, 0.7 to 1.2 M ammonium sulphate, and 100 mM sodium phosphate buffer pH 6.6 to 6.9, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | glycogenin | 8-10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.7-1.2 (M) | |
| 3 | 1 | reservoir | sodium phosphate | 100 (mM) | pH6.6-6.9 |
