1LKY
Structure of the wild-type TEL-SAM polymer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-14 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.752, 60.291, 62.318 |
| Unit cell angles | 116.21, 98.89, 98.65 |
Refinement procedure
| Resolution | 500.000 * - 2.300 |
| R-factor | 0.23 * |
| Rwork | 0.232 |
| R-free | 0.27200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ji7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.140 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 * | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.087 | 0.335 * |
| Number of reflections | 53910 | |
| Completeness [%] | 97.5 | 95.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 5% PEG 4000 2.0 M ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | Bis-Tris propane | 10 (mM) | pH8.5 |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | reservoir | PEG4000 | 5 (%) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.0 (M) |
