1LHW
CRYSTAL STRUCTURE OF THE N-TERMINAL LG-DOMAIN OF SHBG IN COMPLEX WITH 2-METHOXYESTRADIOL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-05-05 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8453 |
Spacegroup name | H 3 2 |
Unit cell lengths | 103.920, 103.920, 84.460 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 * - 1.750 |
Rwork | 0.210 |
R-free | 0.23800 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1d2s |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.520 * |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.790 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.031 | 0.286 |
Total number of observations | 123818 * | |
Number of reflections | 17701 | |
Completeness [%] | 99.4 | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 20 * | HEPES, Calcium chloride, 2-methoxyestradiol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 13 (mg/ml) | |
2 | 1 | drop | sodium HEPES | 50 (mM) | pH7.5 |
3 | 1 | drop | 2.5 (mM) | ||
4 | 1 | drop | 2-MeOE2 | 0.010 (mM) | |
5 | 1 | reservoir | isopropyl alcohol | 20 (%) | |
6 | 1 | reservoir | PEG400 | 10 (%) | |
7 | 1 | reservoir | 100 (mM) | ||
8 | 1 | reservoir | sodium HEPES | 100 (mM) | pH7.5 |