1LFD
CRYSTAL STRUCTURE OF THE ACTIVE RAS PROTEIN COMPLEXED WITH THE RAS-INTERACTING DOMAIN OF RALGDS
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 92 |
Collection date | 1997-09 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.648, 78.256, 87.313 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.061 | 0.061 |
Total number of observations | 129387 * | |
Number of reflections | 30504 | |
<I/σ(I)> | 24.5 | 5.7 |
Completeness [%] | 98.3 | 97.5 |
Redundancy | 4.2 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 50 (mM) | |
2 | 1 | drop | dithiothreitol | 10 (mM) | |
3 | 1 | drop | 5 (mM) | ||
4 | 1 | drop | protein | 15 (mg/ml) | |
5 | 1 | reservoir | PEG8000 | 7-10 (%) | |
6 | 1 | reservoir | MES | 100 (mM) | |
7 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
8 | 1 | reservoir | dithiothreitol | 4 (mM) | |
9 | 1 | reservoir | 5 (mM) |