1LDK

Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF Ubiquitin Ligase Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	170
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.928
Spacegroup name	P 21 21 2
Unit cell lengths	219.378, 50.529, 158.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.200 *
R-factor	0.289 *
Rwork	0.289
R-free	0.33100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007 *
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.100
Rmerge	0.091 *	0.332 *
Total number of observations	123884 *
Number of reflections	30847
Completeness [%]	88.8 *	63.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	4 *	peg4k, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7	4 *	peg4k, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	BTP	100 (mM)
2	1	reservoir	PEG4000	7-9 (%)
3	1	reservoir		200-400 (mM)
4	1	reservoir	dithiothreitol	5 (mM)	pH7.0
5	1	drop	protein	10-20 (mg/ml)