1LCP
BOVINE LENS LEUCINE AMINOPEPTIDASE COMPLEXED WITH L-LEUCINE PHOSPHONIC ACID
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1994-12-08 |
| Detector | XENTRONICS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 130.400, 130.400, 125.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 1.650 |
| R-factor | 0.16 |
| Rwork | 0.160 |
| R-free | 0.19100 |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.700 * |
| Data reduction software | XDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.000 |
| High resolution limit [Å] | 1.650 |
| Rmerge | 0.074 |
| Total number of observations | 766198 * |
| Number of reflections | 144211 |
| Completeness [%] | 97.7 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | blLAP | 7 (mg/ml) | |
| 2 | 1 | drop | L-leucinephosphonic acid | 10 (mM) | |
| 3 | 1 | drop | 0.050 (mM) | ||
| 4 | 1 | drop | 200 (mM) | ||
| 5 | 1 | drop | Tris-HCl | 50 (mM) | |
| 6 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 7 | 1 | reservoir | 0.050 (mM) | ||
| 8 | 1 | reservoir | MPD |
