1LB1
Crystal Structure of the Dbl and Pleckstrin homology domains of Dbs in complex with RhoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2002-02-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 43 |
| Unit cell lengths | 158.897, 158.897, 151.791 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.800* |
| R-factor | 0.236 * |
| Rwork | 0.236 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Dbs-Cdc42 (pdb code 1KZ7) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | |
| High resolution limit [Å] | 2.800 * | |
| Rmerge | 0.078 * | 0.654 * |
| Total number of observations | 269910 * | |
| Number of reflections | 86038 * | |
| Completeness [%] | 99.1 * | 94.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 18 * | PEG 3350, lithium citrate, Inositol 1,4,5-trisphosphate, pH 8.4, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 10 (mM) | pH8.0 |
| 2 | 1 | drop | 25 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 2 (mM) | |
| 4 | 1 | drop | EDTA | 0.5 (mM) | |
| 5 | 1 | drop | protein | 15 (mg/ml) | |
| 6 | 1 | reservoir | PEG3350 | 17 (%(w/w)) | |
| 7 | 1 | reservoir | lithium citrate | 200 (mM) | |
| 8 | 1 | reservoir | inositol 1,4,5-triphosphate | 1 (mM) |
