1LAM
LEUCINE AMINOPEPTIDASE (UNLIGATED)
Experimental procedure
Temperature [K] | 123 |
Detector technology | AREA DETECTOR |
Collection date | 1995-06-01 |
Detector | XENTRONICS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 131.500, 131.500, 121.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 7.000 - 1.600 |
R-factor | 0.172 |
Rwork | 0.172 |
R-free | 0.20400 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.800 * |
Data reduction software | XDS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.600 * |
Rmerge | 0.070 |
Total number of observations | 357105 * |
Number of reflections | 76376 |
Completeness [%] | 94.4 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.8 | pH 7.8 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | b1LAP | 7 (mg/ml) | |
2 | 1 | drop | 0.050 (mM) | ||
3 | 1 | drop | 200 (mM) | ||
4 | 1 | drop | Tris-HCl | 50 (mM) | |
5 | 1 | reservoir | Tris-HCl | 50 (mM) | |
6 | 1 | reservoir | 0.050 (mM) |