1L9H
Crystal structure of bovine rhodopsin at 2.6 angstroms RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 |
| Unit cell lengths | 96.745, 96.745, 149.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.188 * |
| Rwork | 0.188 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1F88 PDB ENTRY 1HZX |
| RMSD bond length | 0.013 |
| RMSD bond angle | 21.170 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.099 | 0.555 |
| Total number of observations | 124947 * | |
| Number of reflections | 36092 | |
| <I/σ(I)> | 12 | 1 |
| Completeness [%] | 85.6 | 40 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 4 * | Okada, T., (2000) J. Struct. Biol., 130, 73. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES | 30 (mM) | or sodium acetate |
| 2 | 1 | reservoir | beta-mercaptoehtanol | 5-7 (mM) | |
| 3 | 1 | reservoir | 65-90 (mM) | ||
| 4 | 1 | reservoir | HPTO | 0.55-0.75 (%) | |
| 5 | 1 | reservoir | NG | 0.45-0.55 (%) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.84-0.86 (M) |
